Soil DNA Purification Mini Kit - 50 Prep

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26-013G
$275.00

Bead Mill Soil DNA Purification Mini Kit - 50 Prep

The Omni Soil DNA Purification Kit contains silica based spin-capture columns and nontoxic reagents. The optimized reagents are designed specifically for microbial genomic DNA extraction from soil and sediments. After sample lysis using the Omni pre-filled bead beating tubes, microbial DNA is separated from PCR inhibitors such as humic compounds. Microbial DNA is then purified through spin-column capture. Genomic DNA purified using the Omni Soil DNA Kit is ready for downstream applications such as PCR, southern blots and restriction enzyme digestion.

Product Features:

  • Complete DNA purification kit including spin-columns and nontoxic reagents
  • Effective removal of PCR inhibitors including humic compounds
  • Microbial DNA isolation from a variety of organisms including, gram-positive and gram-negative bacteria
  • Isolation of pure DNA from up to 250 mg soil, clay, sediment and compost
  • Rapid sample disruption with 0.7 mm garnet beads
  • Recover up to 25 ug DNA per filter

Illustrated Protocol

Soil DNA


Ordering Information: Unit includes 50 spin-columns, 50 pre-filled bead tubes with 0.7 mm garnet beads, reagents suitable for 50 purifications and instruction guide

Specifications

  • DNA types: Microbial genomic DNA
  • DNA purity: High quality genomic DNA with A260/A280 > 1.8
  • Inhibitor removal: DNA is separated from humic compounds and other PCR inhibitors prior to purification
  • DNA yield: Up to 25 ug per filter
  • Capacity: Capable of microbial DNA extraction from up to 250 mg of soil and sediment
  • Lysis method: Pre-filled 0.7 mm garnet bead tubes on a bead mill homogenizer or vortex mixer
  • Equipment required: Bead mill or vortexer, microcentrifuge, 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol and isopropanol
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