Soil DNA Purification Midi Kit

Soil DNA Purification Midi Kit - 50 Prep

Sku:

26-013B

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Price:

Availability: In stock

Price: $275.00

General Info

Bead Mill Soil DNA Purification Midi Kit - 50 Prep

The Omni Soil DNA Purification Kit contains silica based spin-capture columns and nontoxic reagents. The optimized reagents are designed specifically for microbial genomic DNA extraction from soil and sediments. After sample lysis using glass beads, microbial DNA is separated from PCR inhibitors such as humic compounds. Microbial DNA is then purified through spin-column capture. Genomic DNA purified using the Omni Soil DNA Kit is ready for downstream applications such as PCR, southern blots and restriction enzyme digestion.

Product Features:

  • Complete DNA purification kit including spin-columns and nontoxic reagents
  • Effective removal of PCR inhibitors including humic compounds
  • Microbial DNA isolation from a variety of organisms including, gram-positive and gram-negative bacteria
  • Isolation of pure DNA from up to 1 g soil, clay, sediment and compost
  • Recover up to 25 ug DNA per filter

Illustrated Protocol

Soil DNA


Ordering Information: Unit includes 50 spin-columns, bulk 0.5 mm glass, reagents suitable for 50 purifications and instruction guide. Note: 15 mL conical tubes not included

Specifications

  • DNA types: Microbial genomic DNA
  • DNA purity: High quality genomic DNA with A260/A280 > 1.8
  • Inhibitor removal: DNA is separated from humic compounds and other PCR inhibitors prior to purification
  • DNA yield: Up to 25 ug per filter
  • Capacity: Capable of microbial DNA extraction from up to 1 g of soil and sediment
  • Lysis method: 0.5 mm glass beads on a bead mill homogenizer or vortex mixer
  • Equipment required: Bead mill or vortexer, microcentrifuge, 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol and isopropanol

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