Bacterial RNA Purification Kit

Bacterial RNA Purification Kit - 50 Prep

Sku:

26-011

Qty:

Price:

Availability: In stock

Price: $220.00

General Info

Bacteria RNA Purification Kit - 50 Prep

The Omni Bacteria RNA Purification Kit contains silica based spin-capture columns and nontoxic reagents. The optimized reagents are designed specifically for RNA extraction from bacteria and utilize highly denaturing buffer conditions to inactivate RNase's. After sample lysis the RNA is purified through spin-column capture in less than 60 minutes. RNA purified using the Omni Bacteria RNA Kit is ready for downstream applications such as RT-PCR.

Product Features:

  • Complete RNA purification kit including spin-columns and nontoxic reagents
  • RNA isolation in less than 60 minutes after lysis
  • No organic extractions
  • Suitable for up to 3 X 107 bacteria cells

Illustrated Protocol

Bacterial RNA


Example Results

Total RNA purified using the Omni Bead Mill Bacterial RNA Purification Kit. RNA was extracted from Esherichia coli (E. coli) at 4.4 x 10^8, 2.2 x 10^8, and 1.1 x 10^8 cfu/mL on the Bead Ruptor Elite, in pre-filled 2 mL tubes with 0.5 mm glass beads at 3.1 m/s for 60 sec. Purified RNA was analyzed on the Agilent 2100 Bioanalyzer. Average RNA Integrity Numbers (RIN) was 8.5.



Ordering Information: Unit includes 50 spin-columns, reagents suitable for 50 purifications and instruction guide

Specifications

  • RNA purity: High quality genomic DNA with A260/A280 > 1.8
  • RNA yield: Up to 100 ug
  • Capacity: Capable of RNA extraction from up to 3 X 107 bacteria cells
  • Equipment required: Microcentrifuge, RNase Free 1.5 mL microcentrifuge tubes, water bath, heat block, 100% ethanol

Applications