The extraction and isolation of RNA is an integral part of downstream analyses such as RT-PCR, RT-qPCR, Northern blotting, and
cDNA library construction. The importance of using pure, intact RNA for these processes is well documented and a critical part
of downstream analysis success. It is well known that RNA is sensitive to degradation due mechanical shear, temperature, storage
conditions and freeze-thawing. Furthermore, RNA is highly susceptible to RNAse degradation following release of nucleases
during the tissue disaggregation process. Thus, proper sample handling during the homogenization process is crucial when
performing an RNA based assay.
The Omni Tissue RNA Purification Kit supports rapid RNA purification with reproducible RNA yields and integrity. Extraction
is based on bead based tissue disruption followed by RNA purification on silica spin columns. Herein, we demonstrate the
performance of the Omni Tissue RNA Purification Kit for RNA purification from multiple murine tissues following disaggregation
on the Bead Ruptor 12 Bead Mill Homogenizer. RNA integrity and yield was compared to tissues dissociated through cryomilling
in a mortar & pestle under liquid nitrogen.