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Title:High-Throughput Genomic DNA and Protein Extraction from Multiple Tissue Types using the Bead Ruptor 96 Bead Mill Homogenizer

Authors: B Easparro, J Atwood

Animal

Application Info

Description:

There have been many studies of correlating gene and protein expression from single tissue samples1,2,3,4. Clinical samples can be especially challenging as sample sourcing and quantity are often limited. This is certainly the case for tissue samples of human origin that represent rare disorders. To make the most of rare tissues an ideal extraction method would be suitable for extracting a multitude of analytes, including nucleic acids, proteins and small molecules. Furthermore, the method would be capable of processing multiple samples simultaneously with high reproducibility. The Bead Ruptor 96, Bead Mill Homogenizer is capable of rapid tissue dissruption via bead milling in 96 well plates, tubes or cryomilling in stainless steel chambers. Herein, we evaluate the Bead Ruptor 96 for high-throughput tissue dissruption for the purification of genomic DNA and proteins. Genomic DNA integrity is evaluated, and the protein repertoire is analyzed via electrophoresis and bottom up semi-quantitative proteomics.

Goal:

DNA and protein were extracted from rat brain, heart, and small intestines. Proteomic coverage was compared for all tissue types.

High-Throughput Genomic DNA and Protein Extraction from Multiple Tissue Types using the Bead Ruptor 96 Bead Mill Homogenizer

Sample Type:

Animal

Tags:

  • Bead Ruptor 96
  • rat
  • heart
  • brain
  • intestines
  • DNA
  • protein
  • proteomics

Created On:

2017-12-06

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