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In this study, we evaluate the potential for automated bead mill disruption of tissues for isolation of nuclei
from rat tissues and compare nuclei purity, nuclear proteome coverage and protein extraction reproducibility
to the extracts isolated by dounce and rotor stator homogenization. Following each extraction, nuclei were
isolated by centrifugation. Purity was then assessed by both microscopy and western blotting for known
nuclear and cytoplasmic protein markers. Proteins from each extraction method were then analyzed by LC-MS/
MS and protein extraction yields were quantified by spectral counting.
Homogenization is often the first step in a tissue proteome study. It has been shown that the accuracy of proteome studies are largely dependent on the sample preparation method utilized. The Omni International Bead Ruptor 12 offers a fast and reproducible solution for tissue
homogenization resulting in high protein recovery.
Cannabinoid quantification (“Potency Testing”) is the most common analytical method performed by cannabis producers and testing facilities. Producers are required to define the number of cannabinoids in cannabis-based products before release to the market. While there are regional variations in potency testing requirements and more than 60 cannabinoids present in cannabis, producers are typically required to define the quantity of the abundant and psychoactive cannabinoids, Tetrahydrocannabinol (THC), Tetrahydrocannabinolic Acid (THCA), Cannabidiol (CBD), Cannabidiolic Acid (CBDA), Cannabigerol (CBG), and Cannabinol (CBN) (Figure 1). 1-2
The most common method for cannabis potency testing is to mill the flower or edibles to create a homogenous mixture in the presence of an organic solvent such as methanol. Following centrifugation to pellet debris, the supernatant is further diluted prior to analysis by reverse phase HPLC or mass spectrometry. While the analytical methods are well defined and easily automated, the sample disaggregation process is low throughput and often tedious. Herein, we evaluate the utility of the Prep 96 automated homogenizer for the extraction of cannabinoids from a spiked cannabis analog.
Proteomic profiling attempts to elucidate not only protein repertoire, but the structure and function of proteins. Post genome sequencing, scientists are now focusing on studying what proteins are decoded from the genome.
Molecular analysis of soil DNA offers a direct solution for detecting microorganisms residing in soil and for studying microbial diversity. Isolation of DNA from soils is often challenging because of the presence of many contaminants, like humic acid, that can interfere with the extraction process and are inhibitory to several downstream applications. An ideal DNA extraction method should effectively eliminate inhibitory substances and maximize DNA yields. The main objective of this study was to compare the performance of Omni International’s Soil DNA Kit (26-013G/B) to that of Company M’s Soil DNA Isolation Kit in terms of DNA yield and quality, as well as amplification potential and sensitivity of detection using real-time PCR.