Application: Bacteria - Leishmania infantum from human serum
Recommended Product: Omni TH tissue homogenizer
Cell pellets were washed and resuspended in 10 ml of lysis buffer. The lysate was maintained on ice for 20 min. The cell suspension was further disrupted with a rotor stator homogenizer, and four cycles of sonification using an ultrasonic homogenizer (sonicator) of 10 s each.
Probe: 7mm Probe
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