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Application: Bacteria - Streptococcus pneumoniae cells
Recommended Product: Omni Ruptor 400 or 4000 Summary:
Cell pellets were frozen at
−80°C and then thawed at room temperature; suspended in 2 ml of spheroplast
buffer (pH 7.5); and incubated at 4°C with constant mixing for 40 min.
Spheroplasts were sedimented by centrifugation at 12,000 × g for 5 min and
suspended in 2 ml sterile water containing 10 mM EDTA and 1 mM PMSF.
Suspensions were sonicated five times for 20 s with an ultrasonic homogenizer
(sonicator) equipped with a microtip on a setting of 4.
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