Application: Bacteria - Streptococcus pneumoniae cells
Recommended Product: Omni Ruptor 400 or 4000
Cell pellets were frozen at −80°C and then thawed at room temperature; suspended in 2 ml of spheroplast buffer (pH 7.5); and incubated at 4°C with constant mixing for 40 min. Spheroplasts were sedimented by centrifugation at 12,000 × g for 5 min and suspended in 2 ml sterile water containing 10 mM EDTA and 1 mM PMSF. Suspensions were sonicated five times for 20 s with an ultrasonic homogenizer (sonicator) equipped with a microtip on a setting of 4.
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