Application: Male and female flies from D. melanogaster
Recommended Product: Omni Bead Ruptor 24
Depending on fly size, 50 or 70 μl of 6 M urea in 50 mM Tris/HCl, pH 6.8 and approx. 50 or 70 mg of glass beads, respectively, were added to the tubes. Lysis was performed in a bead ruptor (bead mill) for 2 × 15 min at 30 Hz at ambient temperature. After a short centrifugation, supernatants were recovered and subjected to two consecutive centrifugation steps at 14,000 rpm to remove insoluble debris.
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