Application: Myo2p/Cdc4p/Rlc1p (Myo2) in Schizosaccharomyces pombe cells
Recommended Product: Omni Bead Ruptor 24
Cells were lysed by glass bead beating with a bead ruptor (bead beater). The lysate was centrifuged at 500 g for 5 min to remove unlysed cells and beads and further centrifuged at 100,000 g for 45 min to remove insoluble matter.
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