To analyse the expression of the VP2 fusion protein by xylose-induced rLc393:pPG611.1-VP2, overnight cultures grown in basal MRS medium supplemented with xylose were collected by centrifugation at 12 000 g for 10 min. The pellets were washed twice with sterile 50 mm Tris-Cl, pH 8·0 and lysed in a bead ruptor (bead-beater) by vigorous shaking. The lysates were centrifuged at 15 000 g for 10 min and the supernate were examined using 10% sodium dodecyl sulphate–polyacrylamide gel electrophoresis.

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