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Application: Human breast cancer lymph nodes – RNA isolation
Recommended Product: Omni TH tissue homogenizer Summary:
Total cellular RNA was isolated from control lymph nodes and lymph nodes from breast cancer subjects using a guanidinium thiocyanate-phenol-chloroform solution. Lymph node specimens were removed from −70°C storage and immediately weighed without allowing the tissue to thaw. SLN were processed separately from ALN. A maximum of 3 ALN were pooled together into 1 sample, while SLN were analyzed individually. Tissue (≤0.15 g) was then homogenized in 1 mL of RNA STAT-60 using a rotor stator homogenizer (polytron), and total RNA was isolated
Probe: G7-95ST For a PDF re-print of the full article, enter your name and e-mail address below: |
