Sputum samples were collected into sterile containers on ice, weighed, and [3H]-LTB4, [3H]-LTC4, [3H]-LTD4, and [3H]-LTE4 (2 nCi) added before homogenization in 4 vol ethanol for 2 min in a rotor stator homogenizer (ultra-turrax).

Probe: 7mm saw tooth or Omni Tip disposable probes

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