Application: Human HeLa S3 (glucocorticoid receptor) cells
Recommended Product: Omni TH or THQ tissue homogenizer
Cells were pelleted and resuspended in an equal volume of ice-cold 11 mM Tris-HCl/EDTA pH 8.3 and homogenized by using a prechilled rotor stator homogenizer (ultra-turrax).
Probe: 7mm saw tooth or Omni Tip disposable probes
For a PDF re-print of the full article, enter your name and e-mail address below: