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Application: Human HeLa S3 (glucocorticoid receptor) cells

Recommended Product: Omni TH or THQ tissue homogenizer

Summary:

Cells were pelleted and resuspended in an equal volume of ice-cold 11 mM Tris-HCl/EDTA pH 8.3 and homogenized by using a prechilled rotor stator homogenizer (ultra-turrax). 

Probe: 7mm saw tooth or Omni Tip disposable probes

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