Application: Human tissue and cell lines
Recommended Product: Omni TH or THQ tissue homogenizer
Tissues were homogenized with a rotor stator homogenizer (ultra-turrax) in 10 vol of sodium acetate (pH 5.5). The homogenates were then sonicated and finally extracted for 20 min at 4 degrees Celsius. The supernatants were employed after centrifugation (20,000 g for 15 min).
Probe: 7mm saw tooth or Omni Tip disposable probes
For a PDF re-print of the full article, enter your name and e-mail address below: