Thylakoids were isolated with Barley segments (3 g, apical 5 cm of primary leaves) were cut into 5 mm^2 pieces and immersed in 25 ml of ice-cold homogenization buffer. This mixtures was homogenized in a rotor stator homogenizer (ultra-turrax) operating at 75% line voltage for two 5-s bursts.

Probe: 7mm saw tooth or Omni Tip disposable probes

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