One L of cell culture suspension was harvested during the log phase of growth and homogenized with 200 mL of deoxygenated 66 micrometers K-phosphate (pH 7) for 15 min using a rotor stator homogenizer (ultra-turrax) (3000 rpm) because Oscillatoria cells are difficult to break open.

Probe: 7mm saw tooth or Omni Tip disposable probes

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