The tissue was filtered and washed with 50 mL of substrate-free buffer, homogen ized with 70% acetone with a rotor stator homogenizer (ultra-turrax) (20,000 rpm), and centriduged for 10 min at 5000g and the supernatant evaporated to the aqueous phase.

Probe: 7mm saw tooth or Omni Tip disposable probes

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