Application: Mouse ileal longitudinal muscle layers
Recommended Product: Omni TH or THQ tissue homogenizer
After incubation, isoprenaline (1 μm) was added and allowed to act for 5 min, and then the muscle strips were quickly frozen in liquid nitrogen and homogenized in 6% trichloroacetic acid solution with a rotor stator (polytron) homogenizer.
Probe: 7mm saw tooth or Omni Tip disposable probes
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