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Application: HEK293 cells expressing human recombinant P2X7 receptors and U-2 OS cells
Recommended Product: Omni TH or THQ tissue homogenizer Summary:
Cell pellets from up to 10 T175 flasks of cells were resuspended in 35 ml EDTA buffer (pH 7.4 at 4°C) and homogenised using a rotor stator (polytron) homogenizer (2 × 10 s bursts at full speed).
Probe: 7mm saw tooth or Omni Tip disposable probes For a PDF re-print of the full article, enter your name and e-mail address below: |
