Application: Guinea pig and human recombinant 5-HT4 receptors
Recommended Product: Omni TH or THQ tissue homogenizer
Cells were harvested by gentle mechanical agitation and then centrifugation (1200 g, 5 min). The pellets were resuspended in HEPES buffer, pH 7.4 and homogenized with a rotor stator (polytron) homogenizer (setting 19.2 × 10 s) on ice.
Probe: 7mm saw tooth or Omni Tip disposable probes
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