After extensive dialysis to eliminate the surfactants, proteoliposomes were loaded with 4-methyllumbelliferylphosphate in 10 mM KCl, 10 mM HEPES, 125 mM saccharose (pH 7.4), by sonication (25%, 22 seconds on ice) using an ultrasonic homogenizer (sonicator).

Probe: OR-T-156 or Omni Tip Plastic Probes

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