The culture was centrifuged (10,000 × g, 30 min, 4°C), and the pellets were washed twice in phosphate-buffered saline (PBS; pH 7.2) with 0.005 M MgCl2 (10,000 × g, 30 min, 4°C), resuspended in PBS, and sonicated 20 times for 15 s each time on ice water in an ultrasonic homogenizer (sonicator) at the maximum microtip setting.

Probe: OR-T-156

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