Application: Mouse spinal cords
Recommended Product: Omni TH or THQ tissue homogenizer and Omni Ruptor 250 ultrasonic homogenizer
Dorsal half of the spinal cord lumbar region enlargement (L4–L6) were homogenized with 10 mM PBS (pH 7.4) in a rotor stator (polytron) homogenizer and then sonicated on ice for 1 minute (3 times, 20 seconds each time).
Probe: 7mm saw tooth or Omni Tip plastic probes
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