Application: S. cerevisiae or C. albicans (ceels) fungi
Recommended Product: Omni TH or THQ tissue homogenizer
Frozen cells were ground into a powder with a mortar and pestle in liquid nitrogen to facilitate cell disruption. The cell powder was resuspended in 4 ml of lysis buffer provided in the kit and homogenized for 2 min at 30-s intervals at 25,000 rpm using a rotor stator (polytron) homogenizer.
Probe: 7mm saw tooth or Omni Tip plastic probes
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