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Application: S. cerevisiae or C. albicans (ceels) fungi

Recommended Product: Omni TH or THQ tissue homogenizer

Summary:

Frozen cells were ground into a powder with a mortar and pestle in liquid nitrogen to facilitate cell disruption. The cell powder was resuspended in 4 ml of lysis buffer provided in the kit and homogenized for 2 min at 30-s intervals at 25,000 rpm using a rotor stator (polytron) homogenizer. 

Probe: 7mm saw tooth or Omni Tip plastic probes

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