Application: Human dopamine receptors
Recommended Product: Omni TH or THQ tissue homogenizer
Tris buffer (pH 7.4) was added to the thawed cell pellet (20 ml per 109 cells) and the cell suspension homogenised using a rotor stator homogenizer (ultra-turrax) (6×5 s bursts, 5 s between bursts at 3/4 maximum speed).
Probe: 7mm saw tooth or Omni Tip plastic probes
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